Skip to main content

High nucleotide and amino acid sequence similarities in tumour necrosis factor-α amongst Indian buffalo (Bubalus bubalis), Indian cattle (Bos indicus) and other ruminants

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

Summary

Tumour necrosis factor-α (TNF-α) mRNA from Indian water buffalo (Bubalus bubalis) and Indian cattle (Bos indicus) was reverse transcribed and amplified using reverse transcriptase–polymerase chain reaction (RT-PCR). The nucleotide sequences of cDNAs were determined after cloning into pGEM-T-Easy vector (Promega, Madison, WI) and compared with reported nucleotide sequences of TNF-α cDNA from other species. The nucleotide sequences of TNF-α from Indian cattle revealed significantly high similarities at nucleotide (99.2%) and amino acid (100%) levels with those of cattle (Bos taurus; Zebu). The sequences from buffalo had 98.4% nucleotide and 99.1% amino acid similarities with Indian cattle, indicating functional cross-reactivity. One amino acid deletion at position 63 and one substitution (A→P) at position 64 were observed in buffalo compared with Indian cattle. The amino acid deletion at position 63 was predicted due to differences in pre-mRNA splicing.

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1365-2370.2004.00471.x

Affiliations: 1:  National Biotechnology Centre and 2: Division of Biochemistry and Food Sciences, Indian Veterinary Research Institute, Izatnagar, India.

Publication date: 2004-08-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more