Detection of polymorphisms in the tumour necrosis factor alpha candidate gene in sheep

$48.00 plus tax (Refund Policy)

Download / Buy Article:



Primers based on GenBank sequences of the ovine tumour necrosis factor (TNF)-α gene were designed to amplify a 273-bp fragment comprising part of the fourth exon and the 3′ untranslated region (UTR) of the ovine TNF-α gene. Five different single-strand conformational polymorphism (SSCP) patterns were detected in a number of unrelated animals and three different alleles were identified and sequenced. These alleles differed in one deletion and one single nucleotide polymorphism (SNP) and were named TNF*01, TNF*02 and TNF*03. These alleles corresponded to three sequences previously characterized by other groups. In the population analysis, no significant differences were found in the frequencies of the Latxa and Rasa breeds. This is the first description of allelic variation in the ovine TNF-α gene.

Document Type: Short Communication


Publication date: August 1, 2004

Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more