A novel strategy for unambiguous DPB1 typing
A DPB1 typing method is described that assigns DPB1 alleles into six groups based on polymorphism at amino acid positions 8–9 and 84–87 using sequence-specific priming (SSP). The results obtained allow the selection of primers for a subsequent sequence-specific oligonucleotide (SSO) hybridization procedure which permits DPB1 alleles to be analysed separately in a heterozygote individual. This has greatly reduced the occurrence of typing reaction patterns consistent with multiple combinations of DPB1 alleles seen in other DPB1 typing methods.
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Document Type: Short Communication
Affiliations: Tissue Typing Laboratory, Department of Pathology, The Royal Melbourne Hospital, Parkville, VIC 3050, Australia.
Publication date: 1998-10-01