A novel strategy for unambiguous DPB1 typing

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Abstract:

A DPB1 typing method is described that assigns DPB1 alleles into six groups based on polymorphism at amino acid positions 8–9 and 84–87 using sequence-specific priming (SSP). The results obtained allow the selection of primers for a subsequent sequence-specific oligonucleotide (SSO) hybridization procedure which permits DPB1 alleles to be analysed separately in a heterozygote individual. This has greatly reduced the occurrence of typing reaction patterns consistent with multiple combinations of DPB1 alleles seen in other DPB1 typing methods.

Document Type: Short Communication

DOI: http://dx.doi.org/10.1046/j.1365-2370.1998.00115.x

Affiliations: Tissue Typing Laboratory, Department of Pathology, The Royal Melbourne Hospital, Parkville, VIC 3050, Australia.

Publication date: October 1, 1998

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