DNA typing for BoLA class I using sequence-specific primers (PCR-SSP)
Abstract:The analysis of cattle MHC (BoLA) class I gene expression is an essential component of studies on immune responses and susceptibility to disease. International BoLA workshops have generated data and reagents that allow discrimination of class I molecules at the haplotype level, but progress has been limited by difficulties encountered in defining single alleles. Our aim in this study was to develop a DNA-based system for improved identification of expressed class I alleles, utilizing available cDNA sequences derived from cattle carrying a series of serologically defined class I specificities. This method has allowed more accurate typing of animals for expression of the class I genes present within a small number of haplotypes. The method has also reliably differentiated between allelic variants (identified by prior sequence analysis) and has split existing serological specificities. The data show that MHC class I genes in cattle are more polymorphic than demonstrated by serology and biochemical analysis.
Document Type: Short Communication
Affiliations: 1: Institute for Animal Health, Compton Laboratory, Compton, UK, 2: Department of Veterinary Clinical Studies, Glasgow University, Glasgow, UK, and, 3: Faculty of Veterinary Medicine, University of Utrecht, Netherlands.
Publication date: October 1, 1998