IngentaConnect Subcutaneous adipose tissue expression of tumour necrosis factor-...

Authors: Koistinen H.A.^{1, *}; Bastard J.P.²; Dusserre E.⁴; Ebeling P.¹; Zegari N.⁵; Andreelli F.⁴; Jardel C.²; Donner M.⁵; Meyer L.⁶; Moulin P.³; Hainque B.²; Riou J.P.⁴; Laville M.⁴; Koivisto V.A.¹; Vidal H.⁴

Source: European Journal of Clinical Investigation, Volume 30, Number 4, April 2000 , pp. 302-310(09)

Publisher: Blackwell Publishing

Abstract:

Background

An association with subcutaneous adipose tissue TNF expression and insulin resistance has been suggested in obesity/type-2 diabetes, but this has not been examined directly. In the first part of the study we investigated whether this association is present in 7 lean, 10 obese nondiabetic and 9 type-2 diabetic men. In the second part of the study we examined the relationship between adipose tissue TNF mRNA levels and BMI in 81 nondiabetic subjects spanning a wide range of BMIs.

Methods

Subcutaneous adipose tissue TNF mRNA levels and insulin sensitivity were determined with quantitative RT-competitive PCR and hyperinsulinaemic clamp, respectively.

Results.

Subcutaneous adipose tissue TNF mRNA levels were similar in 7 lean and 10 obese nondiabetic and 9 type-2 diabetic men (P = 0.68), and did not change in response to 240-min hyperinsulinaemia. TNF mRNA levels and insulin sensitivity were not correlated. Unexpectedly, no correlation between TNF mRNA and BMI was found. The relationship between adipose tissue TNF mRNA and BMI was examined further in 31 male and 50 female nondiabetic subjects. The subcutaneous adipose tissue TNF mRNA level correlated with BMI in all subjects (r_S = 0.32, P < 0.01), and in a subgroup analysis in men (r_S = 0.55, P < 0.01) but not in women (r_S = - 0.08). The correlation in men was dependent on a fourfold higher TNF mRNA level in 5 morbidly obese men while there was no difference in TNF mRNA levels in lean or obese men.

Conclusions

Subcutaneous adipose tissue TNF expression does not correlate with insulin sensitivity in nondiabetic or type-2 diabetic men; is not regulated by acute hyperinsulinaemia; and is increased only in morbidly obese men.

Keywords: Cytokine; hyperinsulinaemic clamp; insulin resistance; obesity; tumour necrosis factor-

Language: English

Document Type: Research article

Affiliations: 1: Helsinki University Central Hospital, Finland 2: Hôpital de la Salpétrière, Paris, France 3: Hôpital de l'Antiquaille, Lyon, France 4: Faculté de Médecine R.T.H. Laënnec, Lyon, France 5: EA 2402 Université H. Poincaré-Nancy-1, Nancy, France 6: Service de Diabetologie, Nancy, France *

Subcutaneous adipose tissue expression of tumour necrosis factor- is not associated with whole body insulin resistance in obese nondiabetic or in type-2 diabetic subjects