Integrin mediated adhesion of mononuclear cells from patients with familial hypercholesterolemia

Authors: de Bont N.¹; Geijtenbeek T.B.H.²; Netea M.G.¹; Smilde T.J.¹; Demacker P.N.M.¹; Figdor C.G.²; van der Meer J.W.M.¹; Stalenhoef A.F.H.^{1, *}

Source: European Journal of Clinical Investigation, Volume 29, Number 9, September 1999 , pp. 749-757(9)

Publisher: Wiley-Blackwell

Abstract:

Low-density lipoproteins (LDL) can induce the adhesion of monocytes to endothelial cells. Monocytes of patients with familial hypercholesterolemia (FH) are exposed to high concentrations of LDL, and it has been reported that adhesiveness of these cells in hypercholesterolemic patients is enhanced. We investigated whether LFA-1 or VLA-4 mediated adhesion is altered in FH patients and whether HMG-CoA reductase inhibitors influence this adhesion.

LFA-1 and VLA-4 mediated adhesion to ICAM-1 and VCAM-1 coated beads was investigated using freshly isolated monocytes and T-lymphocytes from patients with homozygous FH, heterozygous FH (before and after cholesterol lowering treatment), and from controls. In addition, the expression of 1- and 2-integrins on these cells was determined.

Both LFA-1 and VLA-4 mediated adhesion and integrin expression of monocytes and CD3⁺ cells from patients with homozygous FH and heterozygous FH was similar to that of monocytes from a control population. Treatment with HMG-CoA reductase inhibitors did not affect the adherence to ICAM-1 or VCAM-1, and did not influence the expression of integrins.

In contrast to studies by others, we demonstrated in the present study that the actual LFA-1 and VLA-4 mediated adhesion of T-lymphocytes and monocytes is not altered in patients with FH.

Keywords: Adhesion; familial hypercholesterolemia; HMG-CoA reductase inhibitors; LFA-1; VLA-4

Language: English

Document Type: Research article

Affiliations: 1: Department of General Internal Medicine 2: Department of Tumour Immunology, University Hospital Nijmegen, the Netherlands *

Publication date: 1999-09-01

• In this: publication
• By this: publisher
• In this Subject: Public Health
• By this author: de Bont N. ;  Geijtenbeek T.B.H. ;  Netea M.G. ;  Smilde T.J. ;  Demacker P.N.M. ;  Figdor C.G. ;  van der Meer J.W.M. ;  Stalenhoef A.F.H.

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers