Authors: Weber, Henriette; Hellmann, Hanjo

Source: FEBS Journal, Volume 276, Number 22, November 2009 , pp. 6624-6635(12)

Publisher: Wiley-Blackwell

Abstract:

In Arabidopsis thaliana, the BTB/POZ-MATH (BPM) proteins comprise a small family of six members. They have been described previously to use their broad complex, tram track, bric-a-brac/POX virus and zinc finger (BTB/POZ) domain to assemble with CUL3a and CUL3b and potentially to serve as substrate adaptors to cullin-based E3-ligases in plants. In this article, we show that BPMs can also assemble with members of the ethylene response factor/Apetala2 transcription factor family, and that this is mediated by their meprin and TRAF (tumor necrosis factor receptor-associated factor) homology (MATH) domain. In addition, we provide a detailed description of BPM gene expression patterns in different tissues and on abiotic stress treatments, as well as their subcellular localization. This work connects, for the first time, BPM proteins with ethylene response factor/Apetala2 family members, which is likely to represent a novel regulatory mechanism of transcriptional control. Structured digital abstract

•   MINT-7262792: BPM1 (uniprotkb: Q8L765) physically interacts ( MI:0915) with RAP2-4 (uniprotkb: Q8H1E4) by two hybrid ( MI:0018)

•   MINT-7262805: BPM1 (uniprotkb: Q8L765) physically interacts ( MI:0915) with RAP2-13 (uniprotkb: Q9LM15) by two hybrid ( MI:0018)

•   MINT-7262749: BPM3 (uniprotkb: Q2V416) physically interacts ( MI:0915) with RAP2-4 (uniprotkb: Q8H1E4) by two hybrid ( MI:0018)

•   MINT-7262764: BPM3 (uniprotkb: Q2V416) physically interacts ( MI:0915) with RAP2-13 (uniprotkb: Q9LM15) by two hybrid ( MI:0018)

•   MINT-7262838, MINT-7262882, MINT-7262898, MINT-7263072: RAP2-4 (uniprotkb: Q8H1E4) binds ( MI:0407) to BPM1 (uniprotkb: Q8L765) by pull down ( MI:0096)

•   MINT-7262911: RAP2-4 (uniprotkb: Q8H1E4) binds ( MI:0407) to BPM2 (uniprotkb: Q9M8J9) by pull down ( MI:0096)

•   MINT-7262935: RAP2-4 (uniprotkb: Q8H1E4) binds ( MI:0407) to BPM3 (uniprotkb: Q2V416) by pull down ( MI:0096)

•   MINT-7262945: RAP2-4 (uniprotkb: Q8H1E4) binds ( MI:0407) to BPM4 (uniprotkb: Q9SRV1) by pull down ( MI:0096)

•   MINT-7262970: RAP2-4 (uniprotkb: Q8H1E4) binds ( MI:0407) to BPM5 (uniprotkb: Q1EBV6) by pull down ( MI:0096)

•   MINT-7262992: RAP2-4 (uniprotkb: Q8H1E4) binds ( MI:0407) to BPM6 (uniprotkb: A1L4W5) by pull down ( MI:0096)

•   MINT-7263095: RAP2-4 (uniprotkb: Q8H1E4) binds ( MI:0407) to RAP2-4 (uniprotkb: Q8H1E4) by pull down ( MI:0096)

•   MINT-7262855: RAP2-13 (uniprotkb: Q9LM15) binds ( MI:0407) to BPM1 (uniprotkb: Q8L765) by pull down ( MI:0096)

•   MINT-7263015: BPM1 (uniprotkb: Q8L765) binds ( MI:0407) to At4g13620 (uniprotkb: Q9SVQ0) by pull down ( MI:0096)

•   MINT-7263047: BPM1 (uniprotkb: Q8L765) binds ( MI:0407) to At4g39780 (uniprotkb: O65665) by pull down ( MI:0096)

Keywords: APETALA; BPM; cullin; proteasome; ubiquitin

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1742-4658.2009.07373.x

Publication date: 2009-11-01

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• In this Subject: Anatomy & Physiology ,  Biology ,  Chemistry (General) ,  Biochemistry
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