IngentaConnect Neuropeptide Y-family receptors Y6 and Y7 in chicken: Cloning, ph...

Authors: Bromée, Torun; Sjödin, Paula; Fredriksson, Robert¹; Boswell, Tim²; Larsson, Tomas A.¹; Salaneck, Erik¹; Zoorob, Rima³; Mohell, Nina¹; Larhammar, Dan¹

Source: FEBS Journal, Volume 273, Number 9, May 2006 , pp. 2048-2063(16)

Publisher: Blackwell Publishing

Abstract:

The peptides of the neuropeptide Y (NPY) family exert their functions, including regulation of appetite and circadian rhythm, by binding to G-protein coupled receptors. Mammals have five subtypes, named Y₁, Y₂, Y₄, Y₅ and Y₆, and recently Y₇ has been discovered in fish and amphibians. In chicken we have previously characterized the first four subtypes and here we describe Y₆ and Y₇. The genes for Y₆ and Y₇ are located 1 megabase apart on chromosome 13, which displays conserved synteny with human chromosome 5 that harbours the Y₆ gene. The porcine PYY radioligand bound the chicken Y₆ receptor with a K_d of 0.80 ± 0.36 nm. No functional coupling was demonstrated. The Y₆ mRNA is expressed in hypothalamus, gastrointestinal tract and adipose tissue. Porcine PYY bound chicken Y₇ with a K_d of 0.14 ± 0.01 nm(mean ± SEM), whereas chicken PYY surprisingly had a much lower affinity, with a K_i of 41 nm, perhaps as a result of its additional amino acid at the N terminus. Truncated peptide fragments had greatly reduced affinity for Y₇, in agreement with its closest relative, Y₂, in chicken and fish, but in contrast to Y₂ in mammals. This suggests that in mammals Y₂ has only recently acquired the ability to bind truncated PYY. Chicken Y₇ has a much more restricted tissue distribution than other subtypes and was only detected in adrenal gland. Y₇ seems to have been lost in mammals. The physiological roles of Y₆ and Y₇ remain to be identified, but our phylogenetic and chromosomal analyses support the ancient origin of these Y receptor genes by chromosome duplications in an early (pregnathostome) vertebrate ancestor.

Keywords: G-protein coupled receptor; NPY; paralogon; PYY; synteny

Document Type: Research article

DOI: 10.1111/j.1742-4658.2006.05221.x

Affiliations: 1: Department of Neuroscience, Unit of Pharmacology, Uppsala University, Sweden 2: Roslin Institute (Edinburgh), Roslin, UK 3: Institut André Lwoff, Unité de Génétique Moléculaire et Intégration des Fonctions Cellulaire, Villejuif, France

You have access to the full text article on a website external to Ingentaconnect.

Please click here to view this article on InterScience.

You may be required to register and activate access on InterScience before you can obtain the full text. If you have any queries please contact onlinehelp@oxon.blackwellpublishing.com

Neuropeptide Y-family receptors Y6 and Y7 in chicken: Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region

Neuropeptide Y-family receptors Y₆ and Y₇ in chicken: Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region