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Rapid differentiation of Ophiostoma ulmi and Ophiostoma novo-ulmi isolates by matrix-assisted-laser-desorption/ionization time-of-flight/time-of-flight mass spectrometry

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The intact yeast phase spores of Ophiostoma ulmi and O. novo-ulmi isolates were analysed by matrix-assisted-laser-desorption/ionization time-of-flight/time-of-flight mass spectrometry. The results clearly showed that isolates of O. ulmi and O. novo-ulmi have different mass peak profiles. The specific mass in m/z may vary slightly. One of the peaks from m/z 7623 to 7630 dominated (in high intensity) mass spectra for all isolates of O. novo-ulmi tested in this study. This specific peak is believed to be CU (cerato-ulmi) protein. However, no specific m/z peak can be found to distinguish between the isolates of ssp. novo-ulmi and ssp. americana. On the contrary, all isolates of O. ulmi tested possessed one of a dominate peak at m/z 7337 to 7342, but none around m/z 7626 as shown in O. novo-ulmi isolates.

Document Type: Research Article


Affiliations: 1: Faculty of Forestry, University of Toronto, Toronto, ON M5S 3B3, Canada 2: Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste Marie, ON P6A 2E5, Canada

Publication date: 2010-02-01

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