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Specific hybridization real-time PCR probes for Phytophthora ramorum detection and diagnosis

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Sudden Oak Death, caused by Phytophthora ramorum, poses a serious threat to native American oaks, and is also present in Europe where it has been isolated from numerous European ornamental plant nurseries. Its proven aggressiveness against plants in the Fagaceae and Ericaceae and the damage it has caused in North America have lead to it being assigned quarantine status. The timely and accurate detection of P. ramorum is a critical aid in the study of the epidemiology and biology of this pathogen. As a regulated organism, the availability of a sensitive and reliable assay is essential when attempting to achieve early detection of the pathogen. In this work, new specific hybridization probes for a real-time PCR amplification method were found to be rapid, robust and labour-saving, and proved suitable for routine use in a molecular diagnostic laboratory.

Document Type: Research Article


Affiliations: 1: Laboratory of Applied Genetics, School of Engineering of Lullier, University of Applied Sciences of Western Switzerland, 150 route de Presinge, 1254 Jussy, Switzerland 2: Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Protection in Horticulture, Braunschweig, Germany 3: Instituto Mediterráneo de Estudios Avanzados, IMEDEA (CSIC-UIB), Miquel Marquès 21, Esporles, Balearic Islands, Spain 4: University of Aberdeen, School of Biological Sciences, Department of Plant and Soil Science, Aberdeen, UK

Publication date: 2007-12-01

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