Summary A protocol has been developed for inoculation of rust disease in Dalbergia sissoo using urediniospores of Maravilia achroa. This is the first time that disease symptoms caused by M. achroa were artificially produced. The method has been successfully employed for screening 15 clones of D. sissoo against rust disease. Clone S-167 was the most resistant remaining free from infection. Clones S-57, S-106 and S-124 were also resistant. Clones S-19, S-24 and S-89 were susceptible.