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Polymerase chain reaction primers for the detection of Ceratobasidium bicorne (uninucleate Rhizoctonia)

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Abstract:

An uninucleate sp. causes a root dieback on Norway spruce () and Scots pine () seedlings in forest nurseries. Primers specific to this pathogen were designed, and they yielded an amplification product from mycelial cultures only when uninucleate sp. was used as a template. Inoculated Norway spruce and Scots pine seedlings with visible symptoms gave a clear and reproducible band. The same band appeared also from Norway spruce seedlings growing in inoculated peat or compost but lacking conspicuous symptoms. One of the benefits of the DNA-based test was its higher sensitivity compared with the traditional isolation. The results are also available more quickly.

French
Résumé

Amorces PCR pour détecter Ceratobasidium bicorne (Rhizoctonia uninucléé)

Un dépérissement des racines de semis d'épicéa commun et de pin sylvestre dans les pépinières forestières est causé par un Rhizoctonia sp. uninucléé. Des amorces spécifiques de ce parasite ont été définies. A partir de cultures mycéliennes, elles n'ont fourni un produit d'amplification qu'avec le Rhizoctonia sp. uninucléé. Des semis d'épicéa commun et de pin sylvestre inoculés et présentant des symptômes ont fourni clairement une bande reproductible. La même bande apparaissait aussi chez des semis d'épicéa commun élevés dans du compost ou de la tourbe inoculée mais qui ne présentaient pas de symptômes évidents. Un des avantages du test basé sur l'ADN est sa sensibilité plus grande que les isolements traditionnels. Les résultats sont aussi plus rapides à obtenir.

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1439-0329.2002.00292.x

Affiliations: 1: Finnish Forest Research Institute, P.O.Box 18, FIN-01301 Vantaa, Finland; 2: Finnish Forest Research Institute, Suonenjoki Research Station, FIN-77600 Suonenjoki, Finland;

Publication date: August 1, 2002

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