Tert expression and telomerase activity in gonads and somatic cells of the Japanese medaka (Oryzias latipes)

$48.00 plus tax (Refund Policy)

Download / Buy Article:

Abstract:

A telomerase reverse transcriptase (Tert) encoding gene was cloned from the testis of the teleost fish Oryzias latipes. The expression pattern of Japanese medaka tert (Ola_tert) was analyzed by reverse transcription–polymerase chain reaction and in situ hybridization. Ola_tert was expressed in embryonic stages as well as in differentiated adult tissues. In tissues of adult medakas the highest tert expression was found in gonads and brain. Furthermore, two different splice variants were described and an Ola_tert antisense transcript was identified. The enzyme activity of Tert was determined using a non-radioactive telomeric amplification protocol and the telomerase activity in various tissues was shown to correlate with the tert expression. The telomerase activity was found to be high in contrast to the generally low activity in differentiated human tissues.
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more