Free Content Cloning and characterization of the tomato karyopherin α1 gene promoter

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Abstract:

The karyopherin α1 (LeKAPα 1) gene of tomato (Lycopersicon esculentum) encodes a receptor involved in nuclear import. To analyze the expression pattern of this gene, a genomic clone containing its upstream region was isolated and sequenced. To study the promoter functionality, a 2170 bp fragment (LM1), was fused to glucuronidase (GUS) and introduced into petunia cells by particle bombardment. For further characterization of the promoter, one inverse and three deletion constructs were studied in cell suspension. To follow its expression in tobacco leaves, transgenic plants expressing GUS under the control of the LM1 promoter were made. Expression of LM1–GUS was largely restricted to actively growing leaf regions, suggesting possible involvement of active cell division and plant growth regulators in LeKAPα 1 expression.

Keywords: expression pattern; kryopherin α; plant growth regulators; promoter analysis; tobacco

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1440-169x.2004.00766.x

Affiliations: 1: Department of Genetics, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan 50250, 2: Department of Plant Sciences Tel Aviv University Tel Aviv 69978, and 3: The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel

Publication date: December 1, 2004

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