Caenorhabditis elegans DAF-21 (HSP90) is characteristically and predominantly expressed in germline cells: Spatial and temporal analysis
Authors: Tadao Inoue1; Katsumi Takamura2; Hisanori Yamae1; Naoto Ise1; Manabu Kawakami1; Yo Tabuse3; Johji Miwa4; Yasunori Yamaguchi1
Source: Development Growth & Differentiation, Volume 45, Number 4, August 2003 , pp. 369-376(8)
Publisher: Blackwell Publishing
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Abstract:
Three monoclonal antibodies against antigens that exist in the Caenorhabditis elegans germ line have previously been described. In the present study, a full-length mRNA for one of these antigens was isolated, and by sequencing its corresponding cDNA, it was predicted that the protein would show a high homology with the 90 kDa heat shock protein (HSP90) in other species, and with the protein of daf-21, a previously identified hsp90 homologue. The spatial and temporal distribution of the antigen (DAF-21) was analyzed in C. elegans, and the localization of daf-21 mRNA, as detected by in situ hybridization, agreed with that detected by the monoclonal antibody. Under normal conditions, daf-21 mRNA is characteristically distributed in postembryonic germ cells derived from Z2 and Z3 cells in both hermaphrodites and males. Under heat stress conditions, however, daf-21 mRNA was not only detected in germ cells, but also apparently expressed all over the body. In addition, the DAF-21 protein seemed to be localized in the perinuclear region of somatic cells.Keywords: Caenorhabditis elegans; germ cell; hsp90; in situ hybridization
Document Type: Research article
DOI: 10.1046/j.1440-169X.2003.00706.x
Affiliations: 1: Biotechnology and 2: Marine Biotechnology, Fukuyama University, Fukuyama 729-0292, 3: Fundamental Research Laboratories, NEC Corporation, Tsukuba 305-8501 and 4: College of Bioscience and Biotechnology, Chubu University, Kasugai 487-8501, Japan
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