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Free Content Involvement of Frizzled-10 in Wnt-7a signaling during chick limb development

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The dorsal ectoderm of the limb bud is known to regulate anterior–posterior patterning as well as dorsal– ventral patterning during vertebrate limb morphogenesis. Wnt-7a, expressed in the dorsal ectoderm, encodes a key molecule implicated in these events. In the present study, chicken frizzled-10 (Fz-10) encoding a Wnt receptor was used to study mechanisms of Wnt-7a signaling during chick limb patterning, because its expression is restricted to the posterior-distal region of the dorsal limb bud. Fz-10 transcripts colocalize with Sonic hedgehog (Shh) in the dorsal side of stages 18–23 chick limb buds. It was demonstrated that Fz-10 interacts with Wnt-7a to induce synergistically the expression of Wnt-responsive genes, such as Siamois and Xnr3, in Xenopus animal cap assays. In the chick limb bud, Fz-10 expression is regulated by Shh and a signal from the dorsal ectoderm, presumably Wnt-7a, but not by signals from the apical ectodermal ridge. These results suggest that Fz-10 acts as a receptor for Wnt-7a and has a positive effect on Shh expression in the chick limb bud.

Keywords: Sonic hedgehog; Wnt-7a; chick limb development; frizzled-10

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1440-169x.2000.00545.x

Affiliations: Department of Molecular Biology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701-0192, Japan.

Publication date: December 1, 2000

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