Skip to main content

Free Content Embryonic cell commitment by a simultaneous alteration in nucleo/cytoplasmic ratio in sibling cells between subsequent cell cycles

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Abstract:

Studies on murine embryonal carcinoma (EC) cell lines have revealed a mechanism for commitment of early embryonic cells. By means of a particular intraclonal cell surface glycoprotein and intermitotic time heterogeneity found in the EC lines used, we have devised a cell cycle model and written a computer program for cell cycle stimulation. In the present investigation experimental tissue culture data obtained from the EC lines were inserted into the computer program and the simulations represent a good fit to experimental data. It is shown that the dynamics of the driving forces in the ‘cell growth cycle’ and the ‘DNA-division cycle’, when assumed to be loosely coupled and analyzed in subsequent cell cycles, reveal a mechanism that can commit the mother cell to impel her daughter cells into the next stage in embryonic development by altering the relationship between those two uncoupled subcycles. Thereby the nucleo/cytoplasmic ratio (DNA/mass) is altered in a similar way in the two daughter cells. The simulations increase the reliability of the model and open up possibilities to test other embryonic cell systems.

Keywords: cell size; daughter cells; differentiation; mitosis

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1440-169X.1996.t01-5-00009.x

Affiliations: 1: Cell and Molecular Analysis, Department of Oncology and Pathology, Karolinska Institute and Hospital, S-171 76 Stockholm, Sweden. 2: Institute of Mathematical and Physical Science, University of Tromsø, Norway.

Publication date: December 1, 1996

bsc/dgd/1996/00000038/00000006/art00009
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more