Serum antigens detected in pars recta luminal fluid and coelomic envelope surrounding Bufo arenarum eggs
Abstract:In anurans, protease activity from the pars recta portion of the oviduct (under regulation by 17-estradiol), is known to cause ultrastructural alterations on the oocyte surface rendering fertilizability. In mammals, the presence of serum proteins in oviductal fluid via transudation is also well known. In the present study we determined the plasma proteins of the anuran Bufo arenarum that are present in pars recta fluid and oocyte extracellular matrix and characterized the 17-estradiol-induced proteins synthesized de novo and secreted into the pars recta lumen. Rabbit polyclonal antibodies against the soluble proteins in pars recta fluid cross-reacted with anuran plasma proteins and with the extracellular matrix of coelomic eggs based on immunoelectrophoresis and immunohistochemistry, respectively. Using radiolabeled leucine in the absence and presence of 17-estradiol, we show that a polypeptide of 66 kDa molecular mass is the principal protein synthesized and secreted into the pars recta lumen.
Document Type: Research Article
Affiliations: Department of Developmental Biology, National Council for Scientific and Technical Research, National University of Tucuman, 4000 Tucuman, Argentina.
Publication date: December 1, 1996