Skip to main content

Free Content Serum antigens detected in pars recta luminal fluid and coelomic envelope surrounding Bufo arenarum eggs

Download Article:

You have access to the full text article on a website external to ingentaconnect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Abstract:

In anurans, protease activity from the pars recta portion of the oviduct (under regulation by 17-estradiol), is known to cause ultrastructural alterations on the oocyte surface rendering fertilizability. In mammals, the presence of serum proteins in oviductal fluid via transudation is also well known. In the present study we determined the plasma proteins of the anuran Bufo arenarum that are present in pars recta fluid and oocyte extracellular matrix and characterized the 17-estradiol-induced proteins synthesized de novo and secreted into the pars recta lumen. Rabbit polyclonal antibodies against the soluble proteins in pars recta fluid cross-reacted with anuran plasma proteins and with the extracellular matrix of coelomic eggs based on immunoelectrophoresis and immunohistochemistry, respectively. Using radiolabeled leucine in the absence and presence of 17-estradiol, we show that a polypeptide of 66 kDa molecular mass is the principal protein synthesized and secreted into the pars recta lumen.

Keywords: Bufo arenarum; extracellular matrix; pars recta fluid; serum

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1440-169X.1996.t01-5-00003.x

Affiliations: Department of Developmental Biology, National Council for Scientific and Technical Research, National University of Tucuman, 4000 Tucuman, Argentina.

Publication date: December 1, 1996

bsc/dgd/1996/00000038/00000006/art00003
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more