Skip to main content

Free Content Two cis-regulatory elements are essential for the muscle-specific expression of an actin gene in the ascidian embryo

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Muscle cells in the anterior and middle part of the ascidian larval tail differentiate in a highly autonomous manner. Because this autonomy is due to so-called muscle determinants in the myoplasm of fertilized eggs, there is a genetic cascade for muscle cell differentiation in ascidian embryos, which begins with muscle determinants and ends with the expression of muscle-specific structural genes. In this genetic cascade, an important research subject is the disclosure of the molecular nature of muscle determinants. We approached this issue by going upstream of the cascade by analyzing transcriptional control mechanisms of muscle-specific structural genes. The HrMA4a actin gene, a muscle-specific structural gene, has been analyzed for this purpose. It has been demonstrated that a short upstream sequence up to −103 bp from the transcription start site is sufficient for the muscle-specific expression of HrMA4a. In the present study, we performed a more detailed analysis using the -galactosidase gene as a reporter. We demonstrated here that, within the proximal region (−103 to −66) of the HrMA4a gene, there are two short sequences essential to the muscle-specificity of HrMA4a promoter, one is 9 bp long (5′-TCGCACTTC-3′) and the other is 13 bp long (5′-GTGATAACAACTG-3′).
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: ascidian embryos; cis-regulatory elements; muscle actin gene

Document Type: Research Article

Affiliations: Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-01, Japan.

Publication date: 1996-10-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more