IngentaConnect Visualization of human gastric ancer with a novel indrared furesc...

Authors: Ito, Susumu¹; Muguruma, Naoki¹; Hayashi, Sigehito¹; Taoka, Sotoko¹; Bando, Terumi¹; Kusaka, Yosihiro¹; Yano, Mitsuyasu¹; Ichikawa, Souichi¹; Hiasa, Aya¹; Omoya, Tosihiro¹; Honda, Hirohito¹; Shimizu, Ichiro¹; Ii, Kunio²; Nakamura, Kazunari³; Takesako, Kazuhiro⁴; Goto, Yosiaki⁵; Shibamura, Seiichi⁶

Source: Digestive Endoscopy, Volume 12, Number 1, January 2000 , pp. 33-36(4)

Publisher: Blackwell Publishing

Abstract:

Background: An indocyanine green (ICG) derivative (ICG N-hydroxy sulfo succinimide ester (ICG-sulfo-OSu)) as an antibody labeling substance suitable for vital immunohistochemical staining, and an appropriate fluorescence imaging system for ICG-sulfo-OSu using infrared (IR) rays has been developed. The aim of this study is to demonstrate the antibody labeling substance at the cancerous tissue using this imaging system.

Methods: ICG-sulfo-OSu-labeled mouse antihuman carcinoembryonic antigen (CEA) monoclonal antibody and the imaging system for an IR fluorescent microscope were employed in this experiment. According to the standardized procedure, paraffin sections of 10 cases of gastric cancer were stained with anti-CEA antibody by the avidin-biotinylated peroxidase complex (ABC) method. Three cases of gastric cancer that stained positive were used for the IR imaging analysis.

Results: The incubation of the gastric cancer specimens with ICG-sulfo-OSu-anti-CEA antibody-complex resulted in positive staining in the lesion spots. Infrared fluorescence was observed in a well-matched coincidence of oxidized 3,3′-diamino-benzidine tetrahydro-chloride (DAB)-positive sites. No chemical luminescence was confirmed in the rest of the neoplastic areas with ICG-sulfo-OSu complex.

Conclusion: Specific antibodies with ICG-sulfo-OSu have a significant ability to label the cancer cells and reflect sufficient amounts of the fluorescence by IR rays. Therefore, ICG-sulfo-OSu is very useful for IR fluorescent endoscopic detection of microcancers by using the immunohistochemical staining method.

Keywords: endoscopic diagnosis; fluorescence marker; infrared fluorescence; infrared fluorescent endoscope; vital immunohistochemical staining

Document Type: Research article

DOI: 10.1046/j.1443-1661.2000.00007.x

Affiliations: 1: Second Department of Internal Medicine and 2: First Department of Pathology, School of Medicine, The University of Tokushima, Tokushima, 3: Olympus Optical Co. Ltd, Tokyo, 4: Dojindo Laboratories, Kumamoto, 5: Basic Technology Research Laboratory, Daiichi R&D Center, Tokyo and 6: **Daiichi Pure Chemicals Co. Ltd, Tokyo, Japan

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Visualization of human gastric ancer with a novel indrared furescent labeling marker of anti-carcinoembryonic antigen in vitro