EFFECT OF HYDROGEN SULPHIDE ON β-AMYLOID-INDUCED DAMAGE IN PC12 CELLS

Authors: Tang, Xiao-Qing; Yang, Chun-Tao; Chen, Jing; Yin, Wei-Lan; Tian, Shao-Wen; Hu, Bi; Feng, Jian-qiang; Li, Yuan-Jian

Source: Clinical and Experimental Pharmacology and Physiology, Volume 35, Number 2, February 2008 , pp. 180-186(7)

Publisher: Wiley-Blackwell

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Abstract:

SUMMARY

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Hydrogen sulphide (H₂S) is a well-known cytotoxic gas. Recently, H₂S has been shown to protect neurons against oxidative stress caused by glutamate, peroxynitrite and HOCl. Considerably lower H₂S levels have been reported in the brain of Alzheimer's disease (AD) patients with accumulation of β-amyloid (Aβ).

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The aim of present study was to explore the cytoprotection by H₂S against Aβ_25-35-induced apoptosis and the molecular mechanisms underlying this effect in PC12 cells.

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Our findings indicated that Aβ_25-35 significantly reduced cell viability and induced apoptosis of PC12 cells, along with dissipation of the mitochondrial membrane potential (MMP) and overproduction of reactive oxygen species (ROS).

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Sodium hydrosulphide (NaHS), an H₂S donor, protected PC12 cells against Aβ_25-35-induced cytotoxicity and apoptosis not only by reducing the loss of MMP, but also by attenuating the increase in intracellular ROS.

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The results of the present study suggest that the cytoprotection by H₂S is related to the preservation of MMP and attenuation of Aβ_25-35-induced intracellular ROS generation. These findings could significantly advance therapeutic approaches to the neurodegenerative diseases that are associated with oxidative stress, such as AD.

Keywords: β-amyloid; apoptosis; cytoprotection; hydrogen sulphide

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1440-1681.2007.04799.x

Publication date: 2008-02-01