IngentaConnect Memory B lymphocytes determine repertoire oligoclonality early af...

Authors: OMAZIC B.; LUNDKVIST I.¹; MATTSSON J.; PERMERT J.¹; NÄSMAN-BJÖRK I.²

Source: Clinical & Experimental Immunology, Volume 134, Number 1, October 2003 , pp. 159-166(8)

Abstract:

SUMMARY

The objective of this study was to investigate if oligoclonality of the Ig repertoire post-haematopoietic stem cell transplantation (HSCT) is restricted to memory B lymphocytes or if it is a general property among B lymphocytes. As a measure of B lymphocyte repertoire diversity, we have analysed size distribution of polymerase chain reaction (PCR) amplified Ig H complementarity determining region 3 (CDR3) in naive and memory B lymphocytes isolated from patients before HSCT and at 3, 6 and 12 months after HSCT as well as from healthy controls. We demonstrate a limited variation of the IgH CDR3 repertoire in the memory B lymphocyte population compared to the naive B cell population. This difference was significant at 3 and 6 months post-HSCT. Compared to healthy controls there is a significant restriction of the memory B lymphocyte repertoire at 3 months after HSCT, but not of the naive B lymphocyte repertoire. Twelve months after HSCT, the IgH CDR3 repertoire in both memory and naive B lymphocytes are as diverse as in healthy controls. Thus, our findings suggest a role for memory B cells in the restriction of the oligoclonal B cell repertoire observed early after HSCT, which may be of importance when considering reimmunization of transplanted patients.

Keywords: B lymphocyte repertoire; haematopoietic stem cell transplantation; IgH gene expression; immune reconstitution

Document Type: Research article

DOI: 10.1046/j.1365-2249.2003.02260.x

Affiliations: 1: Arvid Wretlind Laboratory at the Center for Surgical Science 2: Biovitrum, Stockholm, Sweden

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Memory B lymphocytes determine repertoire oligoclonality early after haematopoietic stem cell transplantation