Skip to main content

Immunocytochemical localization of cyclo-oxygenase isoforms in cultured human airway structural cells

Buy Article:

$43.00 plus tax (Refund Policy)


Cyclo-oxygenase (COX) exists as two isoforms, COX-1, the constitutive isoform, and COX-2, which is inducible by cytokines or inflammatory stimuli and may participate in airway inflammation. Objective

To determine the basal distribution of COX isoforms, and their regulation by interleukin-1 beta (IL-1β), bradykinin (BK) and dexamethasone (Dex) in cultured airway structural cells. Methods

We measured COX-1 and COX-2 in cultured human airway smooth muscle (HASM) cells, MRC5 fibroblasts and normal human epithelial cells (NHBE) using immunocytochemical analysis. Results

The majority of all types of untreated cultured cells expressed COX-1 (75% of HASM, 75% of MRC5 fibroblasts and 72% of NHBE cells). Fibroblasts and smooth muscle cells showed low constitutive COX-2 expression (2 and 8%, respectively) but this was higher in NHBE cells (28%). IL-1β (24 h incubation) or BK (4 h incubation) had no effect on COX-1 expression in any of the cells studied. In contrast, there was a two- and 1.5-fold rise in the percentage of NHBE cells expressing COX-2; a 7.5- and sixfold rise in the percentage of HASM cells expressing COX-2 and a 33.5- and 20.5-fold increase in the percentage of fibroblasts expressing COX-2 after IL-1β or BK treatment, respectively. Pretreatment with dexamethasone abolished IL-1β- and BK-stimulated COX-2 induction in all cells studied. Conclusion

COX-1 is expressed constitutively in human airway fibroblasts, smooth muscle and epithelial cells but epithelial cells also show constitutive expression of COX-2. Both IL-1β and BK induced COX-2 expression in all cells studied and this induction was blocked by dexamethasone. Immunocytochemical techniques can be successfully used to detect the distribution of COX isoforms in cell cultures.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: bradykinin; cyclo-oxygenase; dexamethasone; epithelial cells; fibroblasts; immunocytochemistry; interleukin 1 beta; smooth muscle cells

Document Type: Research Article

Affiliations: Respiratory Medicine Unit, City Hospital, Nottingham, UK

Publication date: 1999-07-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more