Authors: DÍAZ LIFANTE, ZOILA¹; CAMACHO, CRISTINA ANDRÉS¹; VIRUEL, JUAN; CABALLERO, ADORACIÓN CABRERA²

Source: Botanical Journal of the Linnean Society, Volume 159, Number 3, March 2009 , pp. 477-498(22)

Publisher: Wiley-Blackwell

Abstract:

Karyological and genomic in situ hybridization (GISH) approaches provided evidence of the parentage and origin of the hybrid species Narcissus obsoletus. Here, we demonstrate that the putative parental species, N. serotinus L. and N. elegans (Haw.) Spach, recently proposed because of their intermediate morphological traits, have participated in the hybridization process forming this taxon. Karyotype characterization of parental genomes in populations from S Spain and N Morocco has revealed differences in chromosome length and karyotype asymmetry, highlighting their diploid nature. Multicolour GISH on metaphase plates of N. obsoletus, with N. serotinus and N. elegans DNA used as probes, showed differential fluorescent staining of 10 and 20 chromosomes from parental genomes, respectively. Both parental genomes were detected in the allopolyploid, albeit in a duplicated manner. Secondary hybridization between N. obsoletus and N. serotinus was also detected karyologically. Little karyological differentiation between different geographic regions was found in either N. serotinus or N. obsoletus. © 2009 The Linnean Society of London, Botanical Journal of the Linnean Society, 2009, 159, 477-498.

Keywords: Amaryllidaceae; chromosome; cytotaxonomy; genomic in situ hybridization (GISH); Guadalquivir Basin; hybridization; Mediterranean region; Narcissus serotinus; plant evolution; speciation

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1095-8339.2009.00951.x

Affiliations: 1: Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Apdo. correos 1095, 41080-Sevilla, Spain 2: Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Córdoba, Spain

Publication date: 2009-03-01

Related content

• In this: publication
• By this: publisher
• In this Subject: Biology ,  Botany
• By this author: DÍAZ LIFANTE, ZOILA ;  CAMACHO, CRISTINA ANDRÉS ;  VIRUEL, JUAN ;  CABALLERO, ADORACIÓN CABRERA

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers