Reliable detection of clonal IgH/Bcl2 MBR rearrangement in follicular lymphoma: methodology and clinical significance
Authors: Iqbal S.1; Jenner M.J.R.2; Summers K.E.1; Davies A.J.1; Matthews J.3; Norton A.J.4; Calaminici M.4; Rohatiner A.Z.3; Fitzgibbon J.1; Lister T.A.3; Goff L.K.1
Source: British Journal of Haematology, Volume 124, Number 3, February 2004 , pp. 325-328(4)
Publisher: Blackwell Publishing
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Abstract:
Summary The prognostic significance of IgH/Bcl2 rearrangement in follicular lymphoma (FL) remains contentious; polymerase chain reaction (PCR) methodology and tissue source variability may account for some inconsistencies. As IgH/Bcl2 major breakpoint region (MBR) sequences may be found in normal blood, an MBR+ result by conventional PCR in blood/bone marrow may not indicate FL. To establish tumour MBR status, 190 lymphoid tissue samples with histologically evident FL (and therefore >1% tumour cells) were examined by real-time quantifiable PCR; 50% (95/190) had clonal MBR IgH/Bcl2 (MBR was considered clonal when >1%). Overall survival (median = 11·5 years) of MBR+ and MBR- patients was not significantly different.Keywords: follicular lymphoma; major breakpoint region; RQ-PCR
Document Type: Research article
DOI: 10.1046/j.1365-2141.2003.04796.x
Affiliations: 1: Cancer Research UK Medical Oncology Unit, St Bartholomew's Hospital, Charterhouse Square 2: Department of Haematology 3: Department of Medical Oncology 4: Department of Histopathology, St Bartholomew's Hospital, West Smithfield, London, UK
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