IngentaConnect Chromosome anomalies detected by interphase fluorescence in situ ...

Authors: Dewald G.W.¹; Brockman S.R.¹; Paternoster S.F.¹; Bone N.D.²; O'Fallon J.R.³; Allmer C.³; James C.D.⁴; Jelinek D.F.⁵; Tschumper R.C.⁵; Hanson C.A.⁶; Pruthi R.K.²; Witzig T.E.²; Call T.G.²; Kay N.E.²

Source: British Journal of Haematology, Volume 121, Number 2, April 2003 , pp. 287-295(9)

Publisher: Blackwell Publishing

Abstract:

Summary.

Fluorescence in situ hybridization (FISH) was used to detect 6q–, 11q–, +12, 13q–, 17p– and translocations involving 14q32 in interphase nuclei from blood and/or bone marrow from 113 patients with B-cell chronic lymphocytic leukaemia (B-CLL). A total of 87 patients (77%) had a FISH anomaly: 13q– × 1 was most frequent (64%) followed by 13q– × 2 (28%), +12 (25%), 11q– (15%), 17p– (8%) and 6q– (0%). FISH results for blood and bone marrow cells in 38 patients were similar. Purified CD5⁺/CD19⁺ cells from blood were studied in eight patients and results indicate that in some patients not all B cells have FISH anomalies. We used a defined set of hierarchical FISH risk categories to compare FISH results by stable versus progressive disease, age, sex, Rai stage, CD38⁺ expression and IgV_H mutational status. Significant differences in FISH risk distributions were associated with Rai stage, disease status and CD38⁺, but not by age, sex or IgV_H mutational status. To look for baseline factors associated with high-risk disease, multivariate analysis of age, sex, Rai stage, CD38⁺ and disease status versus FISH risk category was performed. Importantly, only CD38⁺ was significantly associated with high-risk FISH categories (+12, 11q– and 17p–) after adjustment for the effects of other variables.

Keywords: B-CLL; Rai stage; FISH; CD38 expression; IgVH mutations

Document Type: Research article

DOI: 10.1046/j.1365-2141.2003.04265.x

Affiliations: 1: Division of Laboratory Genetics, 2: Division of Hematology, 3: Department of Biostatistics, 4: Division of Experimental Pathology, 5: Department of Immunology, and 6: Division Hematopathology Mayo Clinic, Rochester, MN, USA

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Chromosome anomalies detected by interphase fluorescence in situ hybridization: correlation with significant biological features of B-cell chronic lymphocytic leukaemia