C16 laminin peptide increases angiotropic extravascular migration of human melanoma cells in a shell-less chick chorioallantoic membrane assay
As distinct from intravascular dissemination, extravascular migratory metastasis (EVMM) has been described as a potential additional mechanism of melanoma spread in which tumour cells migrate along the external surfaces of vessels. Recent experimental studies strongly suggest a correlation of angiotropism of melanoma cells with EVMM. Angiotropic melanoma cells are linked to the endothelium by an amorphous matrix confirmed to contain laminin. Objectives
To investigate whether laminin plays a role in this extravascular mechanism of tumour spread. Methods
We tested the effect of the C16 laminin peptide on melanoma spread in a shell-less chick chorioallantoic membrane model. Results
After 3 days, green fluorescent protein-expressing melanoma cells were observed spreading along or in the immediate proximity of vessels. The C16 laminin peptide significantly lengthened the distance of extravascular, angiotropic migration of melanoma cells. Histopathology confirmed the angiotropism of melanoma cells without intravasation, compatible with that observed with human angiotropic melanoma. Conclusions
The results of this study suggest that the C16 laminin γ1 chain peptide has angiotropic, extravascular migration-promoting activity on human melanoma cells, and might be a molecular target for preventing melanoma metastasis.
Document Type: Research Article
Affiliations: 1: Departments of Pathology 2: National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, U.S.A. 3: Department of Pathology and Comprehensive Cancer Center, University of Alabama, Birmingham, AL, U.S.A.
Publication date: 2007-10-01