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Elevated levels of eotaxin and interleukin-5 in blister fluid of bullous pemphigoid: correlation with tissue eosinophilia

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Background Bullous pemphigoid (BP) often provokes blood and tissue eosinophilia, which suggests that some chemoattractants modulate the eosinophil infiltration in BP. Eotaxin, a CC chemokine, strongly attracts eosinophils, and interleukin (IL)-5 induces eosinophil differentiation, proliferation and colony formation in vitro. Objectives To examine the correlation between levels of eotaxin and IL-5 and the number of lesional eosinophils, and the expression of eotaxin in BP lesions. Patients/methods In this study we measured eotaxin and IL-5 levels in blister fluid of BP by enzyme-linked immunosorbent assay. We also examined the expression of eotaxin in BP lesions by immunohistochemistry. Results Both eotaxin and IL-5 were detected at high levels in BP blister fluid. Blister fluid eotaxin, but not IL-5 levels, correlated significantly with the number of dermal infiltrating eosinophils. By immunohistochemistry, eotaxin was strongly expressed in epidermal keratinocytes around BP blisters. Conclusions These findings suggest that eotaxin and IL-5 are strongly associated with the tissue eosinophilia of BP. Therapies which aim to inhibit production of eotaxin and IL-5 may improve the inflammation and blister formation in BP.

Keywords: IL-5; bullous pemphigoid; enzyme-linked immunosorbent assay; eosinophils; eotaxin; immunohistochemistry

Document Type: Research Article


Affiliations: 1: Department of Dermatology, Kanto Central Hospital, 6-25-1 Kamiyohga, Setagaya-ku, Tokyo 158-8531, Japan 2: Department of Allergy and Rheumatology, 3: Department of Bioregulatory Function, Faculty of Medicine, University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan 4: Department of Dermatology, Kyushu University School of Medicine, 3-1-1 Maidashi Higashi-ku Fukuoka 812-8582, Fukuoka, Japan

Publication date: 2000-07-01

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