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Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes

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The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs. © 2005 The Linnean Society of London, Biological Journal of the Linnean Society, 2005, 84, 637–641.

Keywords: Mus musculus; house mouse; speciation

Document Type: Research Article


Affiliations: 1: Centre of Integrated Genomics, Institute of Molecular Genetics, Academy Sciences of the Czech Republic, Videnská 1083, CZ-14220 Prague 4, Czech Republic 2: Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany

Publication date: March 1, 2005

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