A corrected space group for Sulfolobus sulfataricus 5′‐deoxy‐5′‐methylthioadenosine phosphorylase II

$48.00 plus tax (Refund Policy)

Download / Buy Article:


5′‐Deoxy‐5′‐methylthioadenosine phosphorylase (MTAP) catalyzes the phosphorolytic cleavage of 5′‐deoxy‐5′‐methylthioadenosine (MTA), a byproduct of polyamine biosynthesis. The Sulfolobus sulfataricus genome encodes two MTAPs. SsMTAP I has broad substrate specifity, accepting guanosine, inosine, adenosine and MTA, while SsMTAP II is specific for┬áMTA. SsMTAP I forms a donut‐shaped hexamer, while SsMTAP II is a hexamer formed from trimers packed face to face. The structure of SsMTAP II was originally determined in┬áspace group P1 (PDB entry ) and showed R32 pseudosymmetry. Post‐analysis using phenix.xtriage showed that the correct space group is C2. Here, the structure refined in space group C2 is reported and the factors that initially led to the incorrect space‐group assignment are discussed.

Document Type: Research Article

DOI: http://dx.doi.org/10.1107/S0907444911051699

Publication date: March 1, 2012

Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more