Skip to main content

Structure of the effector‐binding domain of the arabinose repressor AraR from Bacillus subtilis

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

In Bacillus subtilis, the arabinose repressor AraR negatively controls the expression of genes in the metabolic pathway of arabinose‐containing polysaccharides. The protein is composed of two domains of different phylogenetic origin and function: an N‐terminal DNA‐binding domain belonging to the GntR family and a C‐terminal effector‐binding domain that shows similarity to members of the GalR/LacI family. The crystal structure of the C‐terminal effector‐binding domain of AraR in complex with the effector l‐arabinose has been determined at 2.2 Å resolution. The l‐arabinose binding affinity was characterized by isothermal titration calorimetry and differential scanning fluorimetry; the K d value was 8.4 ± 0.4 µM. The effect of l‐arabinose on the protein oligomeric state was investigated in solution and detailed analysis of the crystal identified a dimer organization which is distinctive from that of other members of the GalR/LacI family.

Document Type: Research Article

DOI: https://doi.org/10.1107/S090744491105414X

Publication date: 2012-02-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more