Construct optimization for studying protein complexes: obtaining diffraction‐quality crystals of the pseudosymmetric PSPC1–NONO heterodimer

$48.00 plus tax (Refund Policy)

Download / Buy Article:

Abstract:

The methodology of protein crystallography provides a number of potential bottlenecks. Here, an approach to successful structure solution of a difficult heterodimeric complex of two human proteins, paraspeckle component 1 (PSPC1) and non‐POU domain‐containing octamer‐binding protein (NONO), that are involved in gene regulation and the¬†structural integrity of nuclear bodies termed paraspeckles is¬†described. With the aid of bioinformatic predictions and systematic screening of a panel of constructs, bottlenecks of protein solubility, crystallization, crystal quality and crystallographic pseudosymmetry were overcome in order to produce crystals that ultimately revealed the structure.

Document Type: Research Article

DOI: http://dx.doi.org/10.1107/S0907444911039606

Publication date: November 1, 2011

Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more