Expression, crystallization and preliminary crystallographic analysis of YciE, a stress protein from Escherichia coli

Authors: Liu, Deqian; Zhao, Yonghong; Fan, Xiuzhen; Sun, Yuan; Fox, Robert O.

Source: Acta Crystallographica Section D, Volume 60, Number 10, October 2004 , pp. 1888-1889(2)

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Abstract:

The stress protein Escherichia coli YciE was overexpressed and purified in three chromatographic steps. Crystals were obtained using PEG 4000 as a precipitant. The YciE protein crystals diffracted to 3.0 Å resolution using a rotating-anode X-ray source. The lattice type is rhombohedral, with unit-cell parameters a = b = 64.2, c = 167.9 Å, α = β = 90, γ = 120°. The crystal belongs to space group R32.

Keywords: YciE; stress response; heat shock

Document Type: Research article

DOI: http://dx.doi.org/10.1107/S0907444904018591

Publication date: 2004-10-01