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Purification and crystallization of the heterodimeric complex of RAR and RXRα ligand-binding domains in the active conformation

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Abstract:

The ligand-binding domains of the retinoid X receptor α (RXRα) and of the retinoic acid receptor  (RAR) were overexpressed separately and copurified in the heterodimeric form. Using a crystallization solution containing sodium formate and PEG 3350 as precipitant, the heterodimer was cocrystallized with the promiscuous ligand 9-cis-retinoic acid (9C-RA) and a 13-residue fragment of the nuclear receptor interaction domain (NID) of the transcriptional coactivator TRAP220. The crystals grew in the trigonal space group P3121, with unit-cell parameters a = b = 115.7, c = 247.2 Å and two heterodimers per asymmetric unit. X-ray diffraction data were collected to 2.9 Å resolution. The structure was solved by molecular replacement and is currently being refined.

Keywords: coactivator; ligand; nuclear receptor; transcription

Document Type: Research Article

DOI: http://dx.doi.org/10.1107/S0907444904009928

Publication date: June 1, 2004

bsc/ayd/2004/00000060/00000006/art00035
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