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Crystallization and preliminary X-ray crystallographic analyses of EcoO109I and its complex with DNA

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EcoO109I is a type II restriction endonuclease that recognizes seven base pairs of the degenerate and discontinuous sequence RGGNCCY. The enzyme and its complex with DNA were successfully crystallized by the hanging-drop vapour-diffusion method using polyethylene glycols as precipitants. The crystal of EcoO109I belongs to space group I4, with unit-cell parameters a = b = 175.5, c = 44.6 Å, and that of the DNA complex belongs to space group P212121, with unit-cell parameters a = 49.1, b = 71.8, c = 203.2 Å. Full sets of X-ray diffraction data from the enzyme and its complex with DNA were collected to 2.4 and 1.9 Å resolution, respectively.
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Keywords: protein–DNA complexes; restriction endonucleases

Document Type: Research Article

Publication date: 01 June 2004

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