Skip to main content

Crystallization and preliminary crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E using an automated crystallization procedure

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

Human centromere-associated protein E, a member of the kinesin superfamily, is a microtubule-dependent motor protein involved in cell division that has been localized transiently to the kinetochore. The protein is thought to be responsible for the correct attachment and positioning of chromosomes to the mitotic spindle during the metaphase. The 312 kDa protein comprises four different domains. In this study, the focus was on the N-terminal motor domain, which includes the ATP-binding site and a region for microtubule binding. Crystals of the CENP-E motor domain have been obtained by high-throughput crystallization screening using an automated TECAN crystallization robot. The crystals (737 × 132 × 79 µm) belong to the space group P21, with unit-cell parameters a = 49.35, b =  83.70, c = 94.16 Å,  = 103.05°. They diffract to 2.1 Å resolution using synchrotron radiation.

Keywords: cell division; kinesin; kinetochore-associated protein; mitosis

Document Type: Research Article

DOI: http://dx.doi.org/10.1107/S0907444904009564

Publication date: June 1, 2004

bsc/ayd/2004/00000060/00000006/art00031
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more