If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Purification, characterization and preliminary crystallographic studies of a novel plant defensin from Pachyrrhizus erosus seeds

$48.00 plus tax (Refund Policy)

Download / Buy Article:


Defensins are small cysteine-rich antimicrobial peptides that are widely distributed in plants, insects and mammals. As potent defenders in protecting plants from pathogenic fungal attack, plant defensins are presumed to play an important role in the innate immunity of plants and are expected to find applications in the production of transgenic crops. A novel plant defensin protein SPE10 from Pachyrrhizus erosus seeds was purified and partially sequenced. Crystallization screening using the hanging-drop vapour-diffusion method resulted in two crystal forms, from one of which a diffraction data set was collected to 0.98 Å resolution. The crystal belongs to space group P21, with unit-cell parameters a = 32.71, b = 28.11, c = 54.85 Å,  = 103.78°. Preliminary crystallographic studies revealed two subunits in the asymmetric unit.

Keywords: antifungal agents; plant defensin

Document Type: Research Article

DOI: http://dx.doi.org/10.1107/S0907444904007395

Publication date: June 1, 2004

Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more