Crystallization and preliminary X-ray crystallographic analysis of the DNA-binding domain of BldD from Streptomyces coelicolor A3(2)

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The N-terminal DNA-binding domain of BldD from Streptomyces coelicolor A3(2) was crystallized by the hanging-drop vapour-diffusion method at 296 K. A 1.8 Å data set has been collected using synchrotron radiation at Pohang Light Source, South Korea. The crystal belongs to the monoclinic space group C2, with unit-cell parameters a = 77.2, b = 31.8, c = 33.6 Å,  = 105.1°. Analysis of the packing density shows that the asymmetric unit probably contains one molecule, with a solvent content of 43.6%.

Keywords: BldD

Document Type: Research Article


Publication date: June 1, 2004

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