Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium

Authors: Nishimoto, Mamoru; Fushinobu, Shinya; Miyanaga, Akimasa; Wakagi, Takayoshi; Shoun, Hirofumi; Sakka, Kazuo; Ohmiya, Kunio; Nirasawa, Satoru; Kitaoka, Motomitsu; Hayashi, Kiyoshi

Source: Acta Crystallographica Section D, Volume 60, Number 2, 1 February 2004 , pp. 342-343(2)

Publisher: Wiley-Blackwell

Buy & download fulltext article:

Price: $48.00 plus tax (Refund Policy)

Abstract:

Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml⁻¹ purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2₁2₁2₁, with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 Å. X-ray diffraction data were collected to 1.80 Å resolution.

Keywords: xylanase B

Document Type: Research article

DOI: http://dx.doi.org/10.1107/S090744490302715X

Publication date: 2004-02-01