Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium

Authors: Nishimoto, Mamoru; Fushinobu, Shinya; Miyanaga, Akimasa; Wakagi, Takayoshi; Shoun, Hirofumi; Sakka, Kazuo; Ohmiya, Kunio; Nirasawa, Satoru; Kitaoka, Motomitsu; Hayashi, Kiyoshi

Source: Acta Crystallographica Section D, Volume 60, Number 2, 1 February 2004 , pp. 342-343(2)

Publisher: Blackwell Publishing

Purchase options

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$47.44 plus tax Refund Policy

More like this?

In this: publication
By this: publisher
In this Subject: Chemistry (General)
By this author: Nishimoto, Mamoru ; Fushinobu, Shinya ; Miyanaga, Akimasa ; Wakagi, Takayoshi ; Shoun, Hirofumi ; Sakka, Kazuo ; Ohmiya, Kunio ; Nirasawa, Satoru ; Kitaoka, Motomitsu ; Hayashi, Kiyoshi

Content Key:

- Free

- New

- Open Access

- Subscribed

- Free Trial

Abstract:

Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml⁻¹ purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2₁2₁2₁, with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 Å. X-ray diffraction data were collected to 1.80 Å resolution.

Keywords: xylanase B

Document Type: Research article

DOI: 10.1107/S090744490302715X