Authors: KAGAMI, Hiroshi¹; YASUDA, Jun²; TAGAMI, Takahiro³; NAITO, Mitsuru⁴; MATSUBARA, Yuko⁴; HARUMI, Takashi⁴; NOGUCHI, Takamasa¹; YAMAMOTO, Yasuhiro¹; TAKAHASHI, Taikai¹; MATSUYAMA, Junichi¹; KOMATSU, Hiroyuki¹; ONO, Tamao¹

Source: Animal Science Journal, Volume 73, Number 6, December 2002 , pp. 453-456(4)

Publisher: Wiley-Blackwell

Abstract:

Effect of the strain combination of the donor and recipient on production efficiency of W-bearing sperm in mixed-sex chimeric testes was analyzed. The combinations of the donors and recipients were White Leghorn (WL) and Rhode Island Red (RIR), and vice versa. Generated mixed-sex chimeras that had the male phenotype at sexual maturity were classified into four groups: (1) a female WL donor and a male RIR recipient; (2) a male WL donor and a female RIR recipient; (3) a female RIR donor and a male WL recipient; (4) a male RIR donor and a female WL recipient. The mean number of W-bearing sperm detected by in situ hybridization among 10 000 sperm observed were 147, 165, 30 and 45 in groups 1, 2, 3 and 4, respectively. The numbers in groups 1 and 2 were both significantly higher than those of groups 3 and 4 (P < 0.05). The combination of a WL donor and a RIR recipient produced W-bearing sperm more efficiently than the reverse combination.

Keywords: chickens; germline development; mixed-sex chimera; W-bearing sperm

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1344-3941.2002.00062.x

Affiliations: 1: Faculty of Agriculture, Shinshu University, Minamiminowa-mura, Nagano-ken, 2: School of Veterinary Medicine, Azabu University, Sagamihara-shi, 3: National Institute of Livestock and Grassland Sciences, Tsukuba Norin Kenkyu Danchi, Ibaraki-ken and 4: National Institute of Agrobiological Sciences, Tsukuba Norin Kenkyu Danchi, Ibaraki-ken, Japan

Publication date: 2002-12-01

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• In this Subject: Zoology
• By this author: KAGAMI, Hiroshi ;  YASUDA, Jun ;  TAGAMI, Takahiro ;  NAITO, Mitsuru ;  MATSUBARA, Yuko ;  HARUMI, Takashi ;  NOGUCHI, Takamasa ;  YAMAMOTO, Yasuhiro ;  TAKAHASHI, Taikai ;  MATSUYAMA, Junichi ;  KOMATSU, Hiroyuki ;  ONO, Tamao

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