Authors: To, K. F.¹; Chan, F. K. L.²; Cheng, A. S. L.²; Lee, T. L.¹; Ng, Y. P.²; Sung, J. J. Y.²

Source: Alimentary Pharmacology & Therapeutics, Volume 15, Number 1, January 2001 , pp. 25-34(10)

Publisher: Wiley-Blackwell

Abstract:

Background: 

The expression of cyclooxygenase (COX) in human gastric ulcers is unknown. Aim: 

To study the expression and cellular localization of cyclooxygenase in human gastric ulcers. Methods: 

A total of 38 surgical gastric ulcer specimens were studied; 20 were Helicobacter pylori-positive and 18 were associated with NSAID use. Twenty non-ulcerated, histologically normal gastric specimens were used as controls. The cellular localization of COX-1 and COX-2 were determined by immunohistochemistry and double immunofluorescence. Cyclooxygenase messenger RNA (mRNA) was measured by reverse transcription-polymerase chain reaction and localized by in situ hybridization. Results: 

In control specimens, COX-1 was detected in stromal cells in the lamina propria. There was focal and weak immunostaining for COX-2 in the foveolar epithelium. At the ulcer edge, COX-1 was significantly increased in lamina propria cells whereas COX-2 was strongly expressed in the hyperplastic foveolar epithelium in H. pylori- and non-steroidal anti-inflammatory drugs (NSAID)-associated ulcers. At the ulcer base, there was strong expression of COX-1 and COX-2 in myofibroblasts, macrophages and endothelial cells in the granulation tissue, irrespective of H. pylori status or NSAID use. Messenger RNA of COX-1 and COX-2 were demonstrated by reverse transcription-polymerase chain reaction. Double immunofluorescence and in situ hybridization confirmed the cellular localization of cyclooxygenase at protein and mRNA levels, respectively. Conclusion: 

Both COX-1 and COX-2 are up-regulated in human gastric ulcers.

Document Type: Research article

Affiliations: 1: Department of Anatomical & Cellular Pathology, The Chinese University of Hong Kong, Hong Kong 2: Department of Medicine & Therapeutics, The Chinese University of Hong Kong, Hong Kong

Publication date: 2001-01-01

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• In this Subject: Pathology ,  Pharmacology ,  Surgery ,  Therapeutics & Alternative Medicine
• By this author: To, K. F. ;  Chan, F. K. L. ;  Cheng, A. S. L. ;  Lee, T. L. ;  Ng, Y. P. ;  Sung, J. J. Y.

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