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Purinergic activation of a leak potassium current in freshly dissociated myocytes from mouse thoracic aorta

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Abstract:

Abstract Aim: 

Exogenous ATP elicits a delayed calcium-independent K+ current on freshly isolated mouse thoracic aorta myocytes. We investigated the receptor, the intracellular pathway and the nature of this current. Methods: 

The patch-clamp technique was used to record ATP-elicited delayed K+ current in freshly dissociated myocytes. Results: 

ATP-elicited delayed K+ current was not inhibited by a ‘cocktail’ of K+ channel blockers (4-AP, TEA, apamin, charybdotoxin, glibenclamide). The amplitude of the delayed K+ current decreased after the reduction of extracellular pH from 7.4 to 6.5. These two characteristics suggest that this current could be carried by the TASK subfamily of ‘twin-pore potassium channels’ (K2P). Purinergic agonists including dATP, but not ADP, activated the delayed K+ current, indicating that P2Y11 is the likely receptor involved in its activation. The PKC activator phorbol ester 12,13-didecanoate stimulated this current. In addition, the PKC inhibitor Gö 6850 partially inhibited it. Real-time quantitative PCR showed that the genes encoding TASK-1 and TASK-2 are expressed. Conclusion: 

Our results indicate that blocker cocktail-insensitive delayed K+ current in freshly dissociated aortic myocytes is probably carried by the TASK subfamily of twin-pore channels.

Keywords: ATP; aorta; mouse; purinergic receptors; twin-pore channels

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1748-1716.2008.01884.x

Affiliations: 1:  Department of Zoology and Animal Biology, University of Geneva, Sciences III, Geneva, Switzerland 2:  Genomics Platform, NCCR Frontiers in Genetics, University of Geneva, CMU, Geneva, Switzerland

Publication date: February 1, 2009

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