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Characterization of ryanodine receptors in rat colonic epithelium

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Abstract:

Abstract Aim: 

Functional evidence suggests the presence of two types of intracellular Ca2+ channels responsible for the release of Ca2+ from Ca2+-stores, i.e. inositol-1,4,5-trisphosphate (IP3R) and ryanodine receptors (RyR), in rat colonic epithelium. Generally, three ryanodine receptor isoforms (RyR1–RyR3) are known; however, the type of RyR at this epithelium is unknown and was the focus of the present study. Methods: 

RyRs were characterized by molecular biological and immunohistochemical methods in the rat colon. Results: 

A transcript of RyR1 was found in mRNA from colonic crypts. In contrast, RyR2 and RyR3 were found in their corresponding reference tissues, but not in the cDNA from colonic crypts suggesting a predominant expression of the RyR1 isoform in this epithelium.

In order to characterize the subcellular localization of RyR1, immunohistochemical experiments were performed. They showed that RyR1 is present in the lamina epithelialis mucosae and smooth muscle cells and is distributed equally along the whole crypt axis with no difference between surface and crypt cells. A double staining with IP3R3, the dominant cytoplasmic isoform of IP3Rs in this epithelium, revealed that there is only little colocalization of the two receptor subtypes within the epithelial cells. Furthermore, the epithelium is equipped with the enzyme CD38 responsible for the production of cyclic adenosine diphosphate ribose, the physiological agonist of RyR.

RyRs are known to be activated by changes in the redox state. The oxidant, monochloramine evoked a ruthenium red-sensitive Ca2+ release all over the crypt axis. This release was unaffected by prior stimulation of IP3 receptors with ATP (and vice versa). Conclusion: 

The present data suggest a functional separation of IP3- and ryanodine receptor-carrying Ca2+ stores in the colonic epithelium.

Keywords: RyR1; ion transport; rat colon; ryanodine receptors

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1748-1716.2007.01802.x

Publication date: 2008-06-01

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