Interstitial cells of Cajal and electrical activity of smooth muscle in porcine ileum

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Abstract:

Abstract Aim: 

To identify the interstitial cells of Cajal in the porcine ileum for the first time immunohistochemically and to examine the electrical properties of intestinal smooth muscle in the same region. Methods: 

In vitro intracellular microelectrode recordings were made from smooth muscle cells in cross-sectional preparations from abattoir-derived healthy porcine ileum. Immunohistochemical labelling of interstitial cells of Cajal was performed using an anti-Kit antibody. Results: 

Slow waves were recorded in the circular muscle layer of all ileal preparations. The mean resting membrane potential of smooth muscle cells was −61.0 ± 1.3 mV. Slow waves had a mean amplitude of 8.5 ± 0.5 mV, a frequency of 9.9 ± 0.1 cycles per minute and a duration of 5.6 ± 0.1 s. A waxing and waning pattern of slow wave activity was occasionally observed. In addition, higher frequency spiking activity associated with contractions was observed in some recordings. The L-type calcium channel blocker nifedipine abolished both the spiking activity and the contractions, but had no significant effect on slow wave characteristics. Current-injection manipulation of the resting membrane potential had no effect on slow wave amplitude, frequency or duration. Kit-immunoreactive interstitial cells of Cajal were identified in the ileal samples and were present in the region of the myenteric plexus and in the circular and longitudinal muscle layers. Conclusion: 

This study recorded slow waves in vitro and demonstrated immunohistochemically the presence of interstitial cells of Cajal in the normal porcine ileum. This study forms a basis for future physiological and pathophysiological comparative studies of intestinal motility.

Keywords: gastrointestinal; ileum; interstitial cells of Cajal; motility; porcine; slow waves

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1748-1716.2006.01589.x

Publication date: July 1, 2006

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