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Oestrogen influence on myogenic satellite cells following downhill running in male rats: a preliminary study

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Abstract:

Abstract Aim: 

This study examined the effect of oestrogen supplementation in rats on myogenic satellite cell quantities in type I and II muscles following eccentric exercise. Methods: 

Gonad intact adult male rats divided into four groups, oestrogen supplemented (25 mg oestrogen pellet) control (EC), oestrogen supplemented, exercised (EE), sham (no oestrogen) control (SC) and sham, exercised (SE). After 1 week of oestrogen exposure the EE and SE animals performed 90 min of intermittent downhill running (5 min running/2 min rest @−13.5° incline and 17 m min−1 speed). Seventy-two hours later exercised (EE and SE) and control (EC and SC) animals were killed and blood samples taken and soleus and white (superficial) vastus muscles surgically removed. Histochemical sections of soleus and white vastus muscles were examined for myogenic satellite cell content by use of Pax7 antibody and for neutrophil content by use of haematoxylin and eosin (H and E) staining procedures. Results: 

Downhill running resulted in significant elevations in satellite cells and neutrophils detected in both soleus and white vastus muscle samples (P < 0.01). Interestingly, oestrogen supplementation resulted in significantly greater (P < 0.01) post-exercise elevations in satellite cells detected in both soleus and white vastus muscle samples compared with sham (no oestrogen) rats. Increases in neutrophils were significantly (P < 0.05) attenuated in oestrogen supplemented rats relative to sham in soleus but not in white vastus muscles. Conclusions: 

Oestrogen supplementation in male rats may have accentuated the 72 h post-downhill running increase in Pax7 detected myogenic satellite cell number in both soleus and white vastus muscles relative to unsupplemented rats. The mechanisms and physiological consequences of this effect are yet to be determined.

Keywords: exercise; muscle damage; muscle repair; neutrophils; oestrogen; satellite cells

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1365-201X.2005.01427.x

Publication date: May 1, 2005

bsc/aps/2005/00000184/00000001/art00008
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