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Changes in signalling molecule levels in 10-day hindlimb immobilized rat muscles

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Abstract:

Abstract Aim: 

Hindlimb immobilization produces similar percentage decreases in muscle mass in the predominantly type I soleus and type II vastus lateralis muscles. Consequently we hypothesized that the percentage changes in potential regulatory molecules for atrophy would be similar in the two muscle fibre types. Methods: 

Therefore, the purpose of the current study was to measure phosphorylated p38 MAPK and JNK, as well as the protein levels of p53, growth arrest and DNA damage-inducible 45 (GADD45), and full-length poly(ADP-ribose) polymerase (PARP) to determine whether their changes in expression in the soleus and vastus lateralis muscles were similar at 10th day of hindlimb immobilization in young rats. Results: 

Unexpectedly, preferential increases in phosphorylation of p38 MAPK and JNK and in the protein levels of p53, GADD45, as well as decreases in full-length PARP occurred in the soleus muscle, while only p38 phosphorylation increased in the white portion of the vastus lateralis muscle at 10th day of hindlimb immobilization. Conclusion: 

Taken together, these results are interpreted to suggest that some of regulatory processes or kinetics in the atrophy of type I and II muscle fibres during limb immobilization may differ at the 10th day of limb immobilization.

Keywords: JNK; growth arrest and DNA damage-inducible 45; p38 MAPK; p53; poly(ADP-ribose) polymerase

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1365-201X.2004.01395.x

Affiliations: Department of Biomedical Sciences, University of Missouri-Columbia, Columbia, MO, USA

Publication date: 2005-02-01

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