Altered sarcoplasmic reticulum function in rat diaphragm after high-intensity exercise

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Abstract:

ABSTRACT

The present study examined the effects of acute high-intensity exercise on Ca2+ uptake and release rates and Ca2+-adenosine triphosphatase (ATPase) activity of the sarcoplasmic reticulum (SR) from the costal diaphragm. The rats were run on a treadmill at an estimated requirement of 100% of maximal O2 consumption until fatigued (average time to exhaustion: 4.79 min). Muscle lactate and inorganic phosphate after exercise were increased by 65% (P < 0.05) and 35% (P < 0.05), respectively. With exercise, Ca2+ uptake and release, which were detected in homogenates using the Ca2+ fluorescent dye indo-1, were decreased by 24% (P < 0.05) and 22% (P < 0.05), respectively. The reduction in Ca2+ uptake was paralleled by decreased activity of SR Ca2+-ATPase in both the absence and presence of Ca2+ ionophore. These findings demonstrate that, in the diaphragm as well as in the locomotor muscles that have been explored in previous studies, the attenuations of the SR function is brought about by acute high-intensity exercise. These changes in the SR of the diaphragm may contribute, at least in part, to deteriorations in exercise tolerance and work productivity resulting from repetitive physical activities.

Keywords: calcium adenosine triphosphatase; calcium release; calcium uptake; fatigue

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1365-201X.2002.01029.x

Affiliations: 1: Institute of Health Sciences and Physical Education, Osaka City University, Osaka-shi, Osaka, Japan 2: Faculty of Integrated Arts and Sciences, Hiroshima University, Higashihiroshima-shi, Hiroshima-ken, Japan 3: School of Medicine, Hiroshima University, Hiroshima-shi, Hiroshima, Japan

Publication date: November 1, 2002

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