Skip to main content

Reduced calcium tolerance in rat cardiomyocytes after myocardial infarction

Buy Article:

$48.00 plus tax (Refund Policy)

Abstract:

ABSTRACT

During ischaemia and reperfusion the intracellular Na+ concentration is elevated in the cardiomyocytes and the cells are depolarized, both favouring reverse mode Na,Ca-exchange loading of the cell with Ca2+. We examined whether cardiomyocytes from rats with congestive heart failure (CHF) and younger rats (HINCX) which both have a high expression of the Na,Ca-exchanger protein (NCX) showed reduced tolerance to extracellular Ca2+. The CHF was induced in Isofluran anaesthetized rats by left coronary artery ligation. Isolated cardiomyocytes were loaded with Fura-2AM and 140 mmNa+ and exposed to 0.05 mmCa2+. Expression of the Na,Ca-exchanger protein was analysed. Fura-2 340/380 ratio rose more rapidly in HINCX and CHF than in SHAM, and the rise was abolished by Ni2+. Hypercontracture developed more frequently in HINCX and CHF than in SHAM cells. The amount of NCX was 54% higher in HINCX and 76% higher in CHF compared with SHAM. Na+-loaded cardiomyocytes from CHF and HINCX rats are more susceptible to Ca2+ overload than SHAM cells because of the increased capacity for Na,Ca-exchange.

Keywords: Na/Ca-exchanger; heart failure; intra/ extracellular ions; ion exchangers; myocardial infarction

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1365-201X.2002.00999.x

Affiliations: Institute for Experimental Medical Research, University of Oslo, Ullevaal Universityhospital, Oslo, Norway

Publication date: August 1, 2002

bsc/aps/2002/00000175/00000004/art00001
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more