Skip to main content

Insulin insensitivity and delayed transcapillary delivery of insulin in oophorectomized rats treated with testosterone

Buy Article:

$43.00 plus tax (Refund Policy)

The importance of transcapillary insulin delivery as a regulated step was explored in an insulin resistant rat model. Oophorectomized female rats were exposed to testosterone (OVX + T) for 8 weeks and examined with insulin clamps, muscle microdialysis, and analyses of insulin distribution kinetics. The results were compared with those obtained in sham-operated control rats. After OVX + T, onset of glucose uptake in skeletal muscle was significantly (P < 0.001–0.05) delayed compared with controls as measured by the glucose infusion rate (GIR) during a euglycaemic, hyperinsulinaemic clamp (5 mU kg–1 min–1). The increase in interstitial insulin concentrations was also significantly (P < 0.05) delayed (15–20% lower) in OVX + T treated rats compared with control rats, but to such a small magnitude that this alone could not explain the late onset of the insulin effect. Skeletal muscle capillary density, examined histochemically, was diminished (P < 0.01–0.001) by 20–25% after treatment with OVX + T compared with control animals, as was the peripheral blood flow (P < 0.05) by 40–45%, measured with the microsphere technique. Insulin binding was reduced in proportion to the reduced (P < 0.01) vascular surface area by OVX + T treatment. Transcapillary transport rate of insulin, measured by comparisons of the kinetics of inulin and insulin spaces in muscle with time, tended (ns) to be lower after OVX + T compared with control rats (30–40%) as a reflection of the lower capillary surface area. The data suggest that the delayed onset of insulin action after OVX + T results from combined defects in the muscle cell at a postreceptor level and, to a lesser extent, from retarded transcapillary delivery of insulin.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: capillaries; insulin sensitivity; microdialysis; muscle; testosterone

Document Type: Research Article

Affiliations: 1: Department of Heart and Lung Diseases and the Wallenberg Laboratory, Göteborg, Sweden 2: Department of Nephrology, University of Lund, Lund, Sweden 3: Department of Internal Medicine and the Lundberg Laboratory for Diabetes Research, Sahlgrenska University Hospital, Sweden

Publication date: 2001-04-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more