Ginsenoside Rg1 promotes bone marrow stromal cells proliferation via the activation of the estrogen receptor-mediated signaling pathway

$48.00 plus tax (Refund Policy)

Download / Buy Article:

Abstract:

Abstract

Aim: To investigate the possible mechanisms of ginsenoside Rg1 promoting bone marrow stromal cell (BMSC) proliferation. Methods: BMSC were isolated from bone marrow of Sprague-Dawley rats and maintained in vitro. After stimulation with 1 mol/L ginsenoside Rg1 for the indicated time, the proliferation ability of BMSC were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and [3 H]-thymidine incorporation assays. The estrogen receptor (ER) binding activity of BMSC was determined by a specific ER antagonist and an ER binding assay. Furthermore, the influence of ginsenoside Rg1 on the expression of ERα was investigated by RT-PCR and Western blotting assays. Results: BMSC proliferation stimulated by 1 mol/L ginsenoside Rg1 can be completely blocked by 1 mol/L ER antagonist ICI 182, 780, or ERα-specific antagonist methylpiperidinopyrazole. Moreover, Rg1 failed to displace the specific binding of [3 H]17 -estradiol to BMSC cell lysates, suggesting that no direct interaction of Rg1 with the ER is needed for its estrogenic effects. In addition, 1 mol/L Rg1 had no effects on the expression of ERα in either the mRNA or protein levels. Conclusion: Our results indicate that ERα is essential for mediating the effects of Rg1 on stimulating BMSC proliferation, which might involve the ligand/receptor-independent activation of ERα.

Keywords: bone marrow stromal cell; estrogen receptor; ginsenoside Rg1; proliferation

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1745-7254.2008.00874.x

Affiliations: 1: Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China 2: Department of Clinical Laboratory Medicine, Nanjing Benq Hospital, Nanjing 210029, China 3: Department of Medicine, Baylor College of Medicine, Houston, Texas, USA 4: Department of Internal Medicine, the Hospital Affiliated with Shanghai Meishan Group, Nanjing 210039, China 5: Department of Pharmacology, Nanjing Medical University, Nanjing 210029, China

Publication date: October 1, 2008

Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more